Loss of fragile WWOX gene leads to senescence escape and genome instability

Induction of DNA damage response (DDR) to ensure accurate duplication of genetic information is crucial for maintaining genome integrity during DNA replication. Cellular senescence is a DDR mechanism that prevents the proliferation of cells with damaged DNA to avoid mitotic anomalies and inheritance of the damage over cell generations. Human WWOX gene resides within a common fragile site FRA16D that is preferentially prone to form breaks on metaphase chromosome upon replication stress. We report here that primary Wwox knockout (Wwox−/−) mouse embryonic fibroblasts (MEFs) and WWOX-knockdown human dermal fibroblasts failed to undergo replication-induced cellular senescence after multiple passages in vitro. Strikingly, by greater than 20 passages, accelerated cell cycle progression and increased apoptosis occurred in these late-passage Wwox−/− MEFs. These cells exhibited γH2AX upregulation and microsatellite instability, indicating massive accumulation of nuclear DNA lesions. Ultraviolet radiation-induced premature senescence was also blocked by WWOX knockdown in human HEK293T cells. Mechanistically, overproduction of cytosolic reactive oxygen species caused p16Ink4a promoter hypermethylation, aberrant p53/p21Cip1/Waf1 signaling axis and accelerated p27Kip1 protein degradation, thereby leading to the failure of senescence induction in Wwox-deficient cells after serial passage in culture. We determined that significantly reduced protein stability or loss-of-function A135P/V213G mutations in the DNA-binding domain of p53 caused defective induction of p21Cip1/Waf1 in late-passage Wwox−/− MEFs. Treatment of N-acetyl-l-cysteine prevented downregulation of cyclin-dependent kinase inhibitors and induced senescence in Wwox−/− MEFs. Our findings support an important role for fragile WWOX gene in inducing cellular senescence for maintaining genome integrity during DDR through alleviating oxidative stress. Supplementary Information The online version contains supplementary material available at 10.1007/s00018-023-04950-1.


Introduction
The maintenance of genome integrity is crucial for normal cell physiology and organism survival.Upon DNA lesion generation, the induction of DNA damage response (DDR) for inhibiting cell cycle progression and promoting DNA repair safeguards the genome integrity from the generation of potentially deleterious mutations [1].The DDR apparatus consists of complicated and sophisticated networks that are required for preserving genetic information and ensuring accurate transmission of DNA through cell generations during replication.If the DNA lesions are beyond repair capacity, the DDR machinery may trigger apoptosis or cellular senescence [2][3][4].
Cellular senescence is a stress response that has been linked with stimulation of genotoxic agents, telomere shortening or dysfunction, ageing, oxidative stress, oncogene activation, and replication stress [3][4][5][6].This phenomenon was first characterized by a durable arrest of the cell cycle in human fibroblasts serially passaged in culture [7].The presence of senescent cells has been inferred from a number of senescence markers, such as persistent growth arrest, enlarged and flattened cell morphology, enhanced senescence-associated β-galactosidase (SA-β-gal) activity, and increased expression of cyclin-dependent kinase (CDK) inhibitors p16 Ink4a and p21 Cip1/Waf1 [2,3,8].
The p16 Ink4a and p53-p21 Cip1/Waf1 signaling cascades are two central activating pathways of cellular senescence [6,[9][10][11].As a barrier to cell proliferation, p16 Ink4a blocks cyclin D•CDK4/6 complex-mediated inactivation of retinoblastoma (RB) family proteins to suppress cell cycle progression.The expression of p16 Ink4a protein is known to increase with ageing in multiple human tissues, and its inactivation by increased CpG island methylation of the p16 Ink4a promoter region is associated with the escape from senescence induction in human epithelial cells [9,12,13].Upon sensing double-stranded DNA breaks, the DDR stimulates a signaling cascade centered around the damage sensor ataxia telangiectasia-mutated (ATM) kinase that activates p53 to elicit cell cycle arrest and to trigger senescence [1,11].After activation, translocation of p53 into the nucleus for the induction of its transcriptional target p21 Cip1/Waf1 is pivotal for the establishment of senescence [11].In turn, p21 Cip1/Waf1 inhibits CDK2-mediated inactivation of RB, thereby preventing the entry of cells into S phase of the cell cycle.Many DNA-damaging stimuli, such as irradiation, chemotherapeutic drugs, or reactive oxygen species (ROS), engage the ATM-p53-p21 Cip1/Waf1 axis [14].Ataxia telangiectasia and Rad3-related protein (ATR) kinase sensing single-stranded DNA breaks during DNA repair or replication stress also activates p53 [15].Depending on the stress or cell type, the p16 Ink4a and p53-p21 Cip1/Waf1 signaling cascades can act either alone or in combination with each other to induce cellular senescence [2].
Common fragile sites are large chromosomal regions that tend to form gaps or breaks during DNA replication.Alterations at chromosomal fragile sites have been implicated as being causative for human cancers [16,17].Interestingly, some genes residing within the chromosomal fragile region encode proteins that are involved in the protection of genome integrity for maintaining cell homeostasis [18][19][20][21][22]. Human WWOX gene is located at the common fragile site FRA16D and encodes WW domain-containing oxidoreductase [23][24][25].Previous studies have shown that WWOX increases the kinase activities of ATM and ATR for facilitating DNA repair [26][27][28].WWOX also interacts with Brca1 to support non-homologous end-joining repair of DNA double-strand breaks [29].Of note, all whole-body homozygous Wwox knockout (Wwox −/− ) mice died by four weeks postnatally [30][31][32].The cause leading to high embryonic or postnatal lethality in Wwox −/− mice is unknown.Early lethal microcephaly syndrome with epilepsy, cerebellar ataxia, mental retardation and profound developmental delay have been observed in pediatric patients with autosomal recessive mutation of WWOX [33][34][35][36][37][38].WWOX deficiency is also associated with human malignancies and neurodegeneration [39,40].However, the molecular pathogenesis that results in the abnormalities in cell homeostasis due to loss of fragile WWOX remains largely unclear.In this study, we show that targeted disruption of Wwox gene in mouse embryonic fibroblasts (MEFs) facilitates their escape from induction of replicative senescence via inactivating both p16 Ink4a and p21 Cip1/Waf1 and leads to loss of genome integrity after serial passages in cell culture.Treatment of cells with N-acetyll-cysteine (NAC) prevents aberrant cell cycle progression and triggers senescence in Wwox −/− MEFs, indicating that oxidative stress confers the senescence escape in cells null for Wwox alleles.

Isolation and culture of MEFs
Whole-body heterozygous Wwox exon 1-targeting knockout (Wwox +/− ) mice were generated and interbred as previously described [31].MEFs isolated from ~ E14.5 fetuses were cultured at 37 °C in 5% CO 2 in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, Waltham, MA, USA).The procedures for animal experiments were carried out in strict accordance with an approved protocol from the Institutional Animal Care and Use Committee of National Cheng Kung University, Taiwan.

Cell line, lentivirus, reagents and plasmids
HEK293T cells obtained from American Type Culture Collection were maintained in DMEM containing 5% FBS at 37 °C.Human dermal fibroblasts were purchased from Lonza (Basel, Switzerland).Lentivirus-mediated gene knockdown was performed as described previously [41] using the sequences listed in Supplementary Table 1.5-Azacytidine (5-Aza) was obtained from MedChemExpress (#HY-10586; Monmouth Junction, NJ, USA).MG132 was purchased from Calbiochem (#474790; Merck & Co., Inc., Rahway, NJ, USA).NAC and chloroquine diphosphate salt were from Sigma-Aldrich (#A8199 and #C6628, respectively; Burlington, MA, USA).Bleomycin, an inducer of DNA strand breaks, was obtained from Nippon Kayaku Co., Tokyo, Japan.The Cell Counting Kit-8 supplied by Dojindo Molecular Technologies, Inc. (Rockville, MD, USA), and hemocytometer were used to determine the numbers of viable cells in cell proliferation assay.
Human p21 Cip1/Waf1 and p53 expression vectors were obtained from Addgene (#20814 and #11770, respectively; Watertown, MA, USA).Full-length murine p16 Ink4a was amplified by polymerase chain reaction (PCR) using the complementary DNA (cDNA) template generated from early-passage wild-type MEFs and the primer set listed in Supplementary Table 1, and cloned into the pcDNA3.1 vector digested with BamHI and EcoRI.To generate the plasmid constructs expressing mutant p53, site-directed mutagenesis was performed using Agilent's QuikChange Lightning kits (Santa Clara, CA, USA) according to the manufacturer's instructions and the mutation-containing primer sequences (Supplementary Table 1).All expression constructs were confirmed by DNA sequencing.The pGL3 luciferase reporter plasmid containing the 564-bp p16 Ink4a promoter sequence (− 869 to − 305) was a generous gift from Dr. Sumit K. Chanda at the Sanford Burnham Prebys Medical Discovery Institute [42].The WWP-Luc (p21 Cip1/Waf1 promoter) construct was obtained from Dr. Bert Vogelstein (#16451; Addgene, Watertown, MA, USA) [43].The pp53-TA-Luc luciferase-based reporter plasmid harboring a p53 response element (#PT3511-5W; Clontech, Mountain View, CA, USA) was used for monitoring p53-mediated signaling in MEFs.For plasmid DNA transfection, cells were electroporated with the above-mentioned construct or a control vector using a BioRad Gene Pulser System (Hercules, CA, USA) as described previously [44], and cultured at 37 °C in 96-well plates or 10-cm dishes.

Flow cytometric analysis
For bromodeoxyuridine (BrdU) labeling, 8 × 10 5 MEFs in 3 ml were cultured overnight in 6-cm dishes, pulsed by the addition of 1 mM BrdU solution (10 μl; BD Pharmingen, Franklin Lakes, NJ, USA) and further incubated at 37 °C for 2 h.Cells were trypsinized and centrifuged at 2000 rpm for 7 min at 4 °C and the supernatants were discarded.After PBS wash, cells were resuspended in 100 μl of BD Cytofix/ Cytoperm buffer, and incubated at 4 °C for 12 h.After wash with BD Perm/Wash buffer, cells were treated with 20 U/ml DNase I at 37 °C for 1 h.The cells were washed with BD Perm/Wash buffer and then stained with FITC-conjugated anti-BrdU (10 μl antibody diluted to 50 μl using BD Perm/ Wash buffer; BD Pharmingen) for 30 min at room temperature in the dark.For propidium iodide (PI) staining, cells were trypsinized, washed with PBS, fixed with 70% ethanol/ PBS for 30 min at 4 °C and centrifuged at 2000 rpm for 10 min at room temperature.After removal of the supernatant, cells were stained with 500 μl of the staining solution containing 40 μg/ml PI (Sigma-Aldrich) and 100 μg/ml RNase A (Roche, Basel, Switzerland) in PBS for 20 min at room temperature in the dark.For dihydroethidium (DHE) staining, the trypsinized MEFs were washed with PBS and stained with 500 μl of DHE staining solution (5 μM in PBS; Invitrogen, Carlsbad, CA, USA) for 20 min at room temperature in the dark.Cells were analyzed using a FACS Calibur (Becton Dickinson, Mountain View, CA, USA) with excitation set at 488 nm.

Immunofluorescence, annexin V staining and micronucleus assay
MEFs on glass coverslips were fixed with 3.7% formaldehyde/PBS at room temperature for 15 min, washed twice with PBS, and incubated with a permeabilization buffer (0.1% saponin, 1% bovine serum albumin and 0.1% NaN 3 in PBS) at 4 °C for 1 h.Cells were stained with anti-γH2AX antibody (#9718, 1:200 dilution in permeabilization buffer; Cell Signaling Technology, Danvers, MA, USA) at 4 °C overnight.After PBS wash, cells were further stained with an Alexa Fluor 594-conjugated donkey anti-rabbit IgG antibody (#A21207, 1:2000 dilution; Invitrogen, Carlsbad, CA, USA) in the dark at room temperature for 2 h.The samples incubated only with secondary antibody were used as negative controls.After PBS wash, the nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; 1 μg/ml in PBS, Sigma-Aldrich) at room temperature for 5 min.After wash, the samples were mounted on a glass slide and the γH2AX-positive cells were visualized under an Olympus fluorescence microscope (FV1000, Tokyo, Japan).For annexin V-binding assay, MEFs cultured in 12-well plates were washed twice with cold PBS and incubated with phycoerythrin-conjugated annexin V (BD #559763) diluted in annexin V-binding buffer for 20 min at room temperature in the dark.The exposure of cell surface phosphatidylserine was examined by fluorescence microscopy.For micronucleus assay, MEFs on glass coverslips were treated with 200 nM bleomycin at 37 °C for 48 h to induce DNA strand breaks.After incubation, cells were fixed with 3.7% formaldehyde/PBS for 15 min at room temperature, washed with PBS and stained with 1 μg/ml DAPI in PBS.The presence of micronuclei was examined by fluorescence microscopy (Olympus, Tokyo, Japan).

Hypoxanthine phosphoribosyltransferase (Hprt) mutation analysis and microsatellite instability assay
Genomic DNA isolated from MEFs using a Quick-DNA Miniprep kit (#D3024; Zymo Research, Irvine, CA, USA) was utilized for PCR detection of Y-chromosomal genes Ube1, Sry and Zfy.Sequences of the primer sets used are shown in Supplementary Table 1.To analyze the mutation rate in X-linked Hprt gene, the MEFs derived from male mouse embryo were seeded at 1.5 × 10 6 cells/dish in 10-cm dishes and cultured with the presence of 5 μg/ml 6-thioguanine (#15774; Cayman, Ann Arbor, MI, USA) at 37 °C for 14 days with medium changed every 3 to 4 days.Cells were stained with 1% crystal violet in 20% methanol for 1 h at room temperature, and the culture dishes were rinsed with water to remove the unbound dye and detached cells.The cell-bound crystal violet was dissolved with 2 ml of acetic 338 Page 4 of 18 acid solution and the eluate (100 μl) was analyzed by the measurement of optical density at 570 nm using a microplate reader (Synergy HT, BioTek, Winooski, VT, USA).
Microsatellite instability in MEFs was tested with five previously described mononucleotide repeats Bat26, Bat30, Bat37, Bat64, and Bat67 [45,46].PCR amplification of the mononucleotide repeats was carried out using MEF genomic DNA (30 ng), a DNA polymerase (Super Therm Gold Master Mix; Bionovas Biotechnology, Toronto, ON, Canada) and the primer sets listed in Supplementary Table 1.The amplified fragments were separated by capillary electrophoresis using a QIAxcel DNA screening kit (Qiagen Life Sciences, Hilden, Germany).

SA-β-gal staining
The SA-β-gal staining kit (#9860; Cell Signaling Technology, Danvers, MA, USA) was utilized to detect senescent cells according to the manufacturer's protocol.In brief, MEFs were seeded and cultured overnight in 24-well plates.Cells were washed twice with cold PBS and fixed with the fixative solution for 15 min at room temperature.After PBS wash, cells were incubated with a staining solution (pH 5.9-6.1)containing 1 mg/ml X-gal and the supplements A and B in an oven set at 37 °C for two days.The blue senescent cells were visualized under a bright-field microscope (Olympus, Tokyo, Japan).

RNA extraction, reverse transcription PCR, real-time PCR and gene expression profiling
RNA samples were extracted using TRIZOL reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instruction.Reverse transcription PCR and quantitative realtime PCR (qPCR) were performed as described previously [44], using the primer pairs listed in Supplementary Table 1.
For microarray analysis, an Agilent Low Input Quick Amp Labeling kit was utilized to amplify the total RNA (0.2 μg) isolated from MEFs of passage one and label with Cy3.The Cy3-labeled cRNA was fragmented to an average length of 50-100 nucleotides and then hybridized to the Agilent SurePrint G3 Mouse GE 8 × 60K microarray at 65 °C for 17 h.After washing and scanning at 535 nm for Cy3, the microarray images were analyzed using a Feature Extraction 10.5.1.1 software (Agilent Technologies, Santa Clara, CA, USA).

Luciferase reporter assay
A control thymidine kinase promoter-driven renilla luciferase reporter plasmid (pRL-TK) and a firefly luciferase reporter plasmid containing a human p16 Ink4a or p21 Cip1/Waf1 promoter sequence or a p53 response element were transfected into MEFs by electroporation.The cells were incubated at 37 °C for 18 h, and the induction of luciferase activities was measured using a dual-luciferase assay kit (Promega, Madison, WI, USA) according to the manufacturer's protocol.

DNA methylation analysis
To quantify the CpG methylation status within p16 Ink4a and p21 Cip1/Waf1 promoters in MEFs, bisulfite conversion of genomic DNA was performed using an EZ DNA methylation kit (Zymo Research) as described previously [47].PCR amplification of the targeted promoter regions was performed using the primers listed in Supplementary Table 1.After in vitro transcription using the PCR products as the template and T7 RNA polymerase, uracil-specific cleavage of the RNA transcripts by RNase A was conducted and the sizes of the resulting fragments were determined by MALDI-TOF mass spectrometry (MassAR-RAY Analyzer, Agena Bioscience, San Diego, CA, USA).The data were analyzed using an EpiTYPER software.

Chromatin immunoprecipitation (ChIP)
Cells in 15-cm dishes were fixed with 1% formaldehyde/ PBS for 15 min at room temperature and collected for centrifugation at 3000 rpm for 2 min at 4 °C.The cell pellets were mixed with a lysis buffer (4 ml) containing 50 mM Tris (pH 8.0), 2 mM EDTA, 0.1% NP40, 10% glycerol and protease inhibitors using a syringe with 26G needle.After centrifugation at 13,000 rpm for 1 min at 4 °C, the pellets were suspended in a buffer (2 ml) containing 50 mM Tris (pH 8.0), 1.6 mM EDTA, 150 mM NaCl, 0.38% NP40, 0.25% SDS and protease inhibitors.By sonication and centrifugation, chromatin fragments in the supernatant with an average size of 500-1000 bp were prepared.Following determination of the protein concentration, an equal amount of the samples (containing 500 μg proteins) was mixed with anti-p53 (1 μl; #2524; Cell Signaling Technology) or a control IgG and the samples were rotated at 4 °C overnight.The mixtures were further incubated with protein A/G beads at 4 °C for 2 h, and the beads were washed with a high salt buffer (20 mM Tris, 2 mM EDTA, 0.5% NP40, 0.1% SDS, 0.5 M NaCl, pH 8.0) once and a low salt buffer (10 mM Tris, 1 mM EDTA, pH 8.0) for three times.The precipitates were incubated with a buffer (500 μl) containing 10 mM Tris, 0.1 mM EDTA and 1% SDS (pH 8.0) at 65 °C overnight, followed by the treatment of proteinase K (100 μg/sample; Abcam, Cambridge, UK) at 50 °C for 1 h.The captured genomic DNA was extracted using phenol/chloroform/isoamyl alcohol and precipitated by ethanol according to the standard protocols.The DNA samples were dissolved in 40 μl TE buffer and subjected to PCR using the primers listed in Supplementary Table 1.

Statistical analysis
Data were presented as the means ± standard deviation (SD).Statistical significance was determined using oneway or two-way ANOVA, one or two-tailed paired t test, or two-tailed t test as described in figure legends.The differences between groups were considered significant when the P values were less than 0.05.

MEFs null for Wwox gene exhibit increased genome instability and aberrant cell growth and apoptosis after serial passages in culture
MEFs freshly isolated from control or Wwox knockout mouse embryos at E14.5 were cultured on dishes and one sixth of the MEFs were passed every 5 days before the cells reached confluent.The cells at passages 1-4 were defined as early-passage MEFs and passages 20-30 with an average of 25 as late-passage MEFs (Fig. 1a).The control MEFs expressing Wwox protein gradually lost their replicative potential after serial passages in cell culture [48][49][50], whereas rapid and robust cell proliferation was observed in the late-passage Wwox −/− MEFs (Fig. 1b).Cell cycle analysis revealed a higher S-phase fraction in late-passage Wwox −/− MEFs compared to the control MEFs (33.1% knockout vs. 14.6% control; Supplementary Fig. 1a).To test whether long-term passage culture of MEFs in Wwox-null status leads to accelerated cell cycle entry, MEFs were synchronized in G0/G1 phase by serum deprivation for 72 h and then stimulated by the addition of serum growth factors.Compared with late-passage control MEFs, we determined increased percentages of cells progressing into S and G2/M phases of the cell cycle in late-passage Wwox −/− MEFs after treatment with serum for 12 and 18 h (Supplementary Fig. 1a).Likewise, a markedly increased population of actively proliferating cells in serum-stimulated late-passage Wwox −/− MEFs was measured by a high extent of BrdU incorporation into the newly synthesized DNA (Fig. 1c).No significant differences were detected between the early-passage Wwox −/− and control MEFs (Fig. 1c, Supplementary Fig. 1a).Intriguingly, we also detected higher amounts of apoptotic cells in late-passage Wwox −/− MEFs than the control cells, as evidenced by the increased proportion of cells in sub G0 population upon cell cycle analysis, binding of annexin V to cell surface phosphatidylserine, and caspase-3 cleavage (Supplementary Fig. 1a-c).
Since some genetically unstable MEFs have both an increased rate of proliferation and a higher level of apoptosis [51], we analyzed whether late-passage Wwox −/− MEFs exhibit the phenotype of genome instability.γH2AX, the phosphorylated form of histone H2AX, is required for the assembly of repair proteins at DNA lesion sites during DDR and is commonly used as a sensitive marker for DNA double-strain breaks and genome instability in the cells [52,53].By immunofluorescent staining, we determined that γH2AX was highly expressed in late-passage Wwox −/− MEFs (Fig. 1d).Moreover, compared with the control cells, an increased extent of bleomycin-induced 338 Page 6 of 18 micronucleus formation, an indicator of chromosomal damages, was detected in late-passage Wwox −/− MEFs (Fig. 1e).To further examine the mutation frequencies in MEFs, 6-thioguanine resistance acquired by X-linked Hprt gene mutations was used in this study as a global mutation indicator [54].PCR detection of murine Y-chromosomal genes Ube1, Sry and Zfy using the primers listed in Supplementary Table 1 was performed for gender typing.After 6-thioguanine treatment, the significantly increased numbers of viable cells in the late-passage Wwox −/− MEF cultures from male mouse embryo revealed a high incidence of Hprt mutations (Supplementary Fig. 2a-c).Replication of microsatellite DNA sequences is error-prone because of DNA polymerase slippage that may create mutations resulting from single base mismatches or short insertions and deletions [45,46].We determined accumulation of length variations in the microsatellite repetitive DNA sequences in late-passage Wwox −/− MEFs using a panel of mononucleotide repeat markers for detecting the phenotype of microsatellite instability (Supplementary Fig. 2d).Together, our results suggest that genome instability in Wwox-null MEFs upon serial passages in culture may lead to accumulation of deleterious DNA lesions and aberrations in cell cycle progression and apoptosis.

Wwox-deficient cells fail to enter cellular senescence
Normal human primary cells undergo a limited number of divisions in culture and enter a quiescent but viable state called replicative senescence [7].Replicative arrest or slowed growth after a limited period of proliferation in primary MEF culture due to DNA damage accumulation has become an experimental model system for finding the molecular cues underlying cellular senescence [50,[55][56][57][58].After 20 passages in culture, the control MEFs displayed moderately increased DDR marker γH2AX (Fig. 1d), and lost their original fibroblast morphology by acquiring a flattened and enlarged feature (Fig. 2a).In stark contrast, the late-passage MEFs null for Wwox still exhibited a spindleshaped appearance (Fig. 2a).Increased SA-β-gal activity was detected in the control, but not Wwox −/− MEFs at late passages (Fig. 2b).We also examined ultraviolet radiationinduced senescence in human HEK293T cells.Compared with the control cells, knockdown of WWOX protein expression significantly blocked the induction of senescence in HEK293T cells (Supplementary Fig. 3).Similar results were observed in WWOX-knockdown human primary dermal fibroblasts (Supplementary Fig. 4a, b).
Moreover, we detected less amounts of p16 Ink4a and p21 Cip1/Waf1 mRNA in late-passage Wwox −/− MEFs (Fig. 2c,  d, Supplementary Fig. 5).However, mRNA levels of Arf and CDK inhibitors p18 in the INK family and p57 in the KIP/ CIP family were increased in late-passage Wwox −/− MEFs, indicating that transcriptional induction of these cell cycle checkpoint proteins by DDR remains intact in these cells (Fig. 2c, d, Supplementary Fig. 5).The protein expression levels of p16 Ink4a , p21 Cip1/Waf1 and p27 Kip1 were increased in the senescent control MEFs after the continuous cell culture process, whereas the late-passage Wwox −/− MEFs had markedly reduced protein levels of these CDK inhibitors in comparison with the late-passage control cells (Fig. 2e).Significantly decreased protein amounts of CDK inhibitors p16 Ink4a and p27 Kip1 were also observed in WWOXknockdown human cells (Supplementary Fig. 4c).DDR has been shown to increase p27 Kip1 protein stability [59,60].

Defective signaling for transcriptional activation of p21 Cip1/Waf1 and CDK inhibitor promoter methylation prevent senescence induction in late-passage Wwox −/− MEFs
To further investigate the mechanism of CDK inhibitor downregulation for senescence escape in late-passage Wwox −/− MEFs, we examined DDR-triggered signaling that stimulates promoter activation of p16 Ink4a and p21 Cip1/Waf1 in the cells.After transfecting a reporter plasmid containing the p16 Ink4a promoter sequence, we detected a higher luciferase activity in late-passage Wwox −/− MEFs than the control cells (Fig. 3a), indicating that the signal for stimulating p16 Ink4a gene activation is enhanced in the cells null for Wwox.Intriguingly, the reporter activity of p21 Cip1/Waf1 promoter showed a robust decrease in late-passage Wwox −/− MEFs in a luciferase reporter assay (Fig. 3a), suggesting that the upstream signal for p21 Cip1/Waf1 induction is defective in these cells.To study whether the decreased p16 Ink4a and p21 Cip1/Waf1 expression in Wwox −/− MEFs is due to promoter hypermethylation, we demonstrated that the reduced protein expression of both p16 Ink4a and p21 Cip1/Waf1 in latepassage Wwox −/− MEFs was reversed by treating the cells with a DNA methylation inhibitor 5-Aza (Fig. 3b).Ectopic expression of p16 Ink4a and/or p21 Cip1/Waf1 in late-passage Wwox −/− MEFs led to increased SA-β-gal activity and suppression of cell growth (Fig. 3c, d), suggesting that the loss of p16 Ink4a and p21 Cip1/Waf1 expression during serial passage in culture is crucial for the senescence escape of Wwox −/− MEFs.

Aberrant p53/p21 Cip1/Waf1 axis results in decreased senescence induction in Wwox −/− MEFs
The induction of p21 Cip1/Waf1 gene expression is majorly regulated by transcription factor p53 in response to various stress stimuli [63].As a reduced signal transduction capacity for inducing p21 Cip1/Waf1 promoter activation in late-passage Wwox −/− MEFs was detected using a reporter assay (Fig. 3a), we further assessed the expression and function of p53.Our results revealed comparable expression levels of p53 338 Page 8 of 18 mRNA between the control and Wwox −/− MEFs (Fig. 4a,  b).After serial passage in culture, an increase in p53 protein level in late-passage control MEFs was associated with robust expression of p21 Cip1/Waf1 protein probably due to the induction of DDR signal (Fig. 4c).Downregulation of , p27 Kip1 and WWOX in Wwox +/− and Wwox −/− MEFs at early-and late-passages were determined by western blotting.β-actin was used as an internal control (Fig. 4e).Upon DNA damage, phosphorylation of human p53 at Ser15/Ser37 or murine p53 at Ser18 by serine/threonine protein kinases ATM, ATR and DNA-PK causes the dissociation of p53 from its key negative regulator, the E3 ubiquitin ligase MDM2, thereby promoting rapid accumulation and functional activation of p53 [64][65][66].Intriguingly, the increased p53 protein expression and phosphorylation at Ser18 in late-passage Wwox −/− MEF clone no.56 failed to induce p21 Cip1/Waf1 expression (Fig. 4d, f).
As Wwox knockout led to genome instability in MEFs (Fig. 1d, e), we analyzed the DNA sequences of the entire p53 coding region, and identified two point mutations in  5a).The first mutation found at nucleotide 638 in this Wwox −/− MEF clone at intermediate and late passages resulted in a change from thymine to guanine which caused a substitution of valine to glycine at position 213 (Fig. 5b).A second guanine-to-cytosine mutation at nucleotide 403 could only be found in this late-passage Wwox −/− MEF clone and led to an alanine-toproline substitution at position 135 (Fig. 5c).Hotspot mutations at the consensus residues A138 and V216 in the DBD of human p53 have been observed in the cancer cells from patients (Fig. 5a) [67,68].No p53 mutations were observed in the control and other Wwox −/− MEF clones.Although a large amount of the mutant p53 protein (A135P/V213G) was present in the nucleus of late-passage Wwox −/− MEF clone no.56, we detected a significantly decreased binding of nuclear p53 to the p53-response region (− 200 to + 98) at p21 Cip1/Waf1 promoter by ChIP assay (Fig. 5d, e).We further analyzed whether the transcriptional activity of the endogenous p53 in late-passage Wwox −/− MEF clone no.56 is defective using a luciferase reporter plasmid carrying a p53-driven promoter.Similar to the finding of p21 Cip1/Waf1 induction shown in Fig. 4c, we determined an increase in the transactivation activity of endogenous p53 in the control MEFs after serial passage culture (Fig. 5f).However, the p53-induced luciferase reporter activity was significantly reduced in late-passage Wwox −/− MEF clone no.56 (Fig. 5f).Ectopic overexpression of GFP-tagged wild-type p53 upregulated p21 Cip1/Waf1 , suppressed cell growth and induced cellular senescence in late-passage Wwox −/− MEFs, as evidenced by western blotting, the use of a cell counting kit and SA-β-gal staining, respectively (Fig. 5g-i).As comparable levels of MDM2 were detected in MEFs of three genotypes, we determined a reduced binding of MDM2 to human mutant p53 protein (A138P/V216G), suggesting that p53 mutations at these residues may protect it from MDM2mediated degradation (Supplementary Fig. 7).Collectively, our results suggest that functional inactivation of cellular p53 by either promoting its proteasomal and lysosomal degradation or loss-of-function mutations is prerequisite for suppressing p21 Cip1/Waf1 expression, thereby leading to the senescence escape of late-passage Wwox −/− MEFs.

ROS suppression by antioxidant treatment leads to senescence induction in late-passage Wwox −/− MEFs
We have previously shown that WWOX translocates to the mitochondria via its SDR domain in response to stress [69,70].Drosophila WWOX has been suggested to regulate aerobic metabolism, mitochondrial respiratory complexes and endogenous ROS generation [71,72].In spite of the fact that ROS generation is a major determinant of cellular senescence [8,73,74], intracellular accumulation of excessive oxidative damages triggered by dramatically increased ROS may lead to epigenetic and gene expression changes, genome instability and uncontrolled cell growth.By microarray analysis, transcriptional induction of a series of genes that participate in redox homeostasis was observed in the newly isolated MEFs from Wwox −/− mouse embryos (Supplementary Fig. 8), suggesting that Wwox deficiency may result in a substantial redox perturbation in cells.By DHE staining and flow cytometric analysis, we detected a substantially increased level of ROS in late-passage Wwox −/− MEFs (Fig. 6a).To test whether the increased ROS generation due to Wwox deficit confers the defective senescence signaling, MEFs were continually maintained in the medium supplemented with antioxidant NAC after isolation from mouse embryos.Interestingly, treatment of cells with NAC during the passage culture prevented microsatellite instability and resulted in senescence induction in the late-passage Wwox −/− MEFs, as evidenced by their increased SA-β-gal activity and morphological changes (Fig. 6b-e).By quantitative analysis of DNA methylation spanning specific CpG islands, p16 Ink4a promoter hypermethylation in late-passage Wwox −/− MEFs was blocked by NAC treatment (Fig. 6f).The downregulation of p16 Ink4a , p21 Cip1/Waf1 and p53 protein expression in late-passage Wwox −/− MEFs was reversed by the presence of NAC (Fig. 6g).In summary, our findings clearly indicate a crucial role of WWOX in the regulation of p16 Ink4a and p21 Cip1/Waf1 signaling and induction of replicative senescence for maintaining genome integrity through inhibiting ROS overproduction in MEFs (Fig. 7).

Discussion
We have established ex vivo MEF passage cultures and demonstrated that WWOX maintains cellular genome integrity under DNA replication stress through induction of cellular senescence.Without WWOX, aberrant duplication of genetic information may cause genome instability and accumulation of deleterious mutations, thereby leading to uncontrolled cell division or death.Most intriguingly, we determined that WWOX blocks ROS-mediated downregulation of p16 Ink4a and p21 Cip1/Waf1 for triggering cellular senescence.
Human WWOX gene resides at the chromosomal fragile site FRA16D that exhibits an increased frequency of breakage upon replication stress and is preferentially unstable during cancer progression [18].Mutations, deletions, loss of heterozygosity, and translocations of WWOX gene have been noted in many types of human cancers [40].Unfavorable clinical outcomes in patients are associated with reduced or absent expression of WWOX in cancer specimens [75].The loss of WWOX expression in hyperplastic or cancer cells upon DDR induction may lead to senescence escape and consequently promote aberrant cell proliferation and genome instability.The resulting genetic alterations accumulate and may include inactivation of tumor suppressors, such as p53, and oncogene amplification and activation that are intimately linked to the proliferative capacity of cells and drive aggressive tumors.
Cellular senescence occurs throughout the life and plays important roles in a variety of physiology and pathological processes, including embryonic development, wound healing, fibrosis, inflammation, ageing, prevention of neoplastic transformation, Alzheimer's disease, atherosclerosis, and type 2 diabetes [3,6,8,14,76].Early lethal microcephaly syndrome with epilepsy, neurodegeneration and profound growth retardation have been reported in the pediatric patients with autosomal recessive homozygous mutation of WWOX [33][34][35][36][37][38].Whole-body Wwox −/− mice succumb to severe neurodevelopmental deficits, impaired hematopoiesis and metabolic disorders, and survive for less than a month [31,32,77].Recent studies have clearly illustrated the connections of defective DDR machinery to congenital neuropathies, neurodegenerative diseases and metabolic dysfunction [78,79].Whether genome instability resulting from dysregulation of the DDR pathways and senescence escape in the cells lacking WWOX interferes with cell physiology and homeostasis of systemic metabolism by causing aberrant cell proliferation or apoptosis in patients and animals remains to be verified.Excessive oxidative damages of cellular macromolecules and supramolecular complexes or activation of specific signaling pathways resulting from overproduction of intracellular ROS in WWOX-null cells may also lead to the deleterious effects and early lethality in patients with loss-of-function mutation in WWOX gene.
Mitochondria play a vital role to orchestrate cellular energy production through oxidative phosphorylation and are a major source of intracellular ROS [74].A previous study has shown that loss of WWOX expression promotes glycolysis and diminishes mitochondrial respiration, suggesting that WWOX may regulate metabolic reprogramming and redox homeostasis in cells [80].WWOX deficiency exacerbates the phenotypic alterations in the developing Drosophila eye due to cellular dysfunction caused by decreased expression of mitochondrial respiratory chain components [71].The cellular dysfunction engendered by knockdown Page 13 of 18 338 of a mitochondrial respiratory complex gene ND42 in larval eye discs is associated with increased ROS and could be rescued by overexpression of antioxidant enzymes [71].Stringent regulation of intracellular ROS levels is crucial for cell fate determination.High ROS levels cause oxidative damage and cell death, whereas low concentrations of ROS may be required for supporting normal cell homeostasis and growth [81,82].WWOX possesses a short-chain alcohol dehydrogenase/reductase domain at its C-terminus and has been suggested to regulate intracellular ROS levels in Drosophila larvae [72].Although no apparent differences in the intracellular ROS levels were detected between the early-passage control and Wwox −/− MEFs, we determined significant increases in the induction of redox-regulatory genes, ROS scavengers in particular, in the freshly isolated Wwox −/− MEFs.Whether perturbation of redox homeostasis due to Wwox loss in early-passage MEFs triggers an initial antioxidative response necessary to buffer the rising ROS and mitigate cellular damage until the scavenging machinery becomes ineffective as a result of excessive ROS accumulation beyond a threshold after passage culture is unclear.Also, the molecular mechanism by which WWOX controls intracellular ROS generation needs further investigation.
ROS overproduction in cells may exhaust the cellular antioxidant capacity and has been suggested to cause deleterious DNA lesions and participate in epigenetic processes, including DNA methylation and histone methylation and acetylation, for altering gene expression [83,84].Hypermethylation of p16 Ink4a gene promoter has been shown to be induced by oxidative stress and promote cancer progression in human esophageal adenocarcinoma [85].We determined that treatment of either 5-Aza or NAC reversed the reduction in protein expression of both p16 Ink4a and p21 Cip1/ Waf1 in late-passage Wwox −/− MEFs, suggesting the involvement of ROS-mediated promoter methylation in the CDK inhibitor downregulation in Wwox-deficient cells during the passage culture.Using a bisulfite conversion approach, we demonstrated that NAC treatment prevented p16 Ink4a promoter hypermethylation in late-passage Wwox −/− MEFs.Nevertheless, the methylation status of p21 Cip1/Waf1 promoter at specific CpG sites showed no significant differences between the control and Wwox −/− MEFs (Supplementary Fig. 9), indicating that the p21 Cip1/Waf1 downregulation in Wwox −/− MEFs is independent of its promoter methylation.Ectopic p16 Ink4a increased p21 Cip1/Waf1 expression in latepassage Wwox −/− MEFs (lane 3 in Fig. 3c), suggesting that p16 Ink4a may act as an upstream effector to trigger p21 Cip1/ Waf1 induction.It is possible that 5-Aza treatment induces p16 Ink4a upregulation, which in turn stimulates p21 Cip1/Waf1 expression in the late-passage Wwox −/− MEFs.Therefore, both p16 Ink4a promoter hypermethylation and p53 deficit or dysfunction contribute to p21 Cip1/Waf1 downregulation in Wwox-deficient MEFs (Fig. 7).How p16 Ink4a cooperates with p53 to regulate p21 Cip1/Waf1 expression in MEFs is unclear.
When sensing the presence of DNA damage or stalled replication fork, CDK inhibitors are induced to arrest cell cycle progression to allow time for repair before the DNA lesion is passed on to daughter cells.Permanent proliferative arrest of cells caused by persistent DDR signaling due to DNA damage accumulation or progressive telomere erosion may lead to cellular senescence.After serial passage culture, increased levels of γH2AX and CDK inhibitors p16 Ink4a , p21 Cip1/Waf1 and p27 Kip1 as well as senescence induction were observed in Wwox-sufficient control MEFs, suggesting that continuous in vitro cell replication may cause genome  56.In c, the change of nucleotide 403 from guanine to cytosine resulted in a substitution of alanine to proline in p53 in the late-passage Wwox −/− MEF clone no.56.d Subcellular protein fractionation and western blotting were performed to examine the nuclear and cytosolic expression of p53 and p21 Cip1/Waf1 in latepassage Wwox +/− and Wwox −/− MEFs (clone no.54 and 56, respectively).Histone H3 and GAPDH were used as nuclear and cytosolic protein controls, respectively.e ChIP assay was conducted using the chromatin isolated from late-passage Wwox +/− and Wwox −/− MEFs (clone no.54 and 56, respectively).The immunoprecipitated chromatin using a control or anti-p53 antibody was analyzed for the presence of p21 Cip1/Waf1 promoter sequence (from − 200 to 98) by real-time PCR using the specific primers shown in Supplementary Table 1.The quantitative results of ChIP assay were normalized by the input control groups.Data are presented as mean ± SD from three independent experiments.***P ≤ 0.005; Two-tailed paired t test.f A reporter construct containing a p53-driven promoter sequence (pp53-TA-Luc) was transfected into MEFs.The thymidine kinase promoter-Renilla luciferase reporter plasmid (pRL-TK) was used as an internal control.Luciferase activities were determined at 16 h after transfection.Data are presented as mean ± SD from three independent experiments.***P ≤ 0.005; Two-tailed t test.g The protein expression of endogenous p21 Cip1/Waf1 (black arrow), p53 and WWOX and ectopic GFP-tagged human p53 (GFP-p53; black arrowhead) in late-passage Wwox +/+ , Wwox +/− , Wwox −/− MEFs (clone no.32-5, 54 and 56, respectively) was examined by western blotting.β-actin was used as an internal control.M.W., molecular weight.h Cell growth rates of late-passage Wwox +/+ , Wwox +/− , Wwox −/− (clone no.32-5, 54 and 56, respectively) and Wwox −/− MEFs expressing ectopic GFP-p53 or GFP protein.N.S. not significant; ***P ≤ 0.005; Two-tailed t test.i SA-βgal staining of late-passage Wwox +/+ , Wwox +/− , Wwox −/− (clone no.g p16 Ink4a , p21 Cip1/Waf1 , p53 and WWOX protein expression was examined in late-passage Wwox +/− , Wwox −/− , and 1 mM NAC-treated Wwox −/− MEFs.β-actin was used as an internal control in western blotting instability and induce DDR signaling in MEFs.ITCH-mediated ubiquitination of WWOX after DNA damage has been shown to be associated with the activation of checkpoint kinases ATM and ATR for induction of DDR signaling [26,27].WWOX also supports non-homologous end-joining repair of DNA double-strand breaks via interacting with Brca1 [29].These findings are in agreement with the role of WWOX as a genome caretaker [28].Without WWOX, successive accumulation of genetic and epigenetic alterations resulting from senescence escape and accelerated cell cycle progression may exacerbate genome instability further in cells with defective DNA repair pathways.The accumulative aberrations may also have a profound impact on cellular signaling and functions and render the WWOX-deficient cells vulnerable to apoptosis. Many WW domain-containing proteins have a functional role in the ubiquitin-proteasome system [86].Tyr33phosphorylated WWOX physically interacts with and stabilizes p53 during tumor necrosis factor, ultraviolet light, and staurosporine-mediated stress responses [87].We detected downregulation of p53 protein expression in the late-passage Wwox −/− MEF clones except the clone no.56 harboring mutant p53 (A135P/V213G).It is possible that WWOX blocks protein degradation of wild-type p53 via preventing its binding to the E3 ubiquitin ligase MDM2 [87].The A135P/V213G mutations in p53 may protect it from degradation in WWOX-deficient cells as a result of the reduced binding of this mutant p53 protein to MDM2.Whether WWOX promotes p27 Kip1 protein stability through inhibiting its phosphorylation at Thr187 and/or ubiquitination by SCF Skp2 is unclear.
Cellular senescence has emerged as an important contributor to tumor suppression, aging and many age-related diseases [2].Targeting WWOX can be exploited in the treatment of senescence-related diseases.Delineation of WWOXregulated signaling pathways upon DDR activation will gain a greater understanding of the pathogenesis of cancers, agerelated diseases and early postnatal lethality due to WWOX loss in humans.The establishment of murine models with tissue-specific knockout for Wwox gene may help us unravel the in vivo function of WWOX during disease development.

Fig. 1
Fig. 1 MEFs null for Wwox gene exhibit increased genome instability and aberrant cell growth after serial passages in culture.a The experimental setup for serial passage culture of MEFs.b Cell growth rates of Wwox +/− and Wwox −/− MEFs at early-and late-passages.***P ≤ 0.005; Two-way ANOVA.c Wwox +/− and Wwox −/− MEFs at early-and late-passages were cultured in a low serum condition for 72 h.After serum starvation, MEFs were treated with 10% FBS for 12 h and pulsed with 10 μM BrdU during the final 2 h of culture.MEFs were fixed, permeabilized, stained with a FITC-conjugated anti-BrdU antibody and analyzed using a FACS Calibur.The percentages of BrdU-positive MEFs were quantified using the Win-

Fig. 4
Fig. 4 Aberrant p53/p21 Cip1/Waf1 axis leads to decreased p21 Cip1/Waf1 induction in late-passage Wwox −/− MEFs.a, b The mRNA expression levels of p53 in MEFs were determined by reverse transcription PCR (a) and quantitative real-time PCR (b).Data are presented as mean ± SD from three independent experiments.N.S. not significant; One-tailed paired t test.c The protein expression of p21 Cip1/Waf1 , p53 and WWOX in Wwox +/− and Wwox −/− MEFs at early-and late-passages was determined by western blotting.The arrow indicates the endogenous WWOX protein (46 kDa) and the asterisk represents a non-specific band arising from the MEF lysates.β-actin was used as a

Fig. 5
Fig. 5 p53 inactivation causes the failure of senescence induction in Wwox −/− MEFs. a The upper panel is the schematic diagram of p53 protein.The amino acid sequence alignment of human and mouse p53 DBD is shown in the lower panel.TAD, transactivation domain; PRD, proline-rich domain; DBD, DNA-binding domain; TD, tetramerization domain; CRD, C-terminal regulatory domain.b, c The sequencing results of p53 gene coding region from Wwox +/− (clone no.54) and Wwox −/− (clone no.56) MEFs at passages 1 (early), 8 (intermediate) and 20 (late) are shown.In b, the nucleotide 638 from the translational start site of murine p53 changed from thymine to guanine, leading to a substitution of valine to glycine at position 213 in the intermediate and late-passage Wwox −/− MEF clone no.56.In c, the change of nucleotide 403 from guanine to cytosine resulted in a substitution of alanine to proline in p53 in the late-passage Wwox −/− MEF clone no.56.d Subcellular protein fractionation and western blotting were performed to examine the nuclear and cytosolic expression of p53 and p21 Cip1/Waf1 in latepassage Wwox +/− and Wwox −/− MEFs (clone no.54 and 56, respectively).Histone H3 and GAPDH were used as nuclear and cytosolic protein controls, respectively.e ChIP assay was conducted using the chromatin isolated from late-passage Wwox +/− and Wwox −/− MEFs (clone no.54 and 56, respectively).The immunoprecipitated chromatin using a control or anti-p53 antibody was analyzed for the presence of p21 Cip1/Waf1 promoter sequence (from − 200 to 98) by real-time PCR using the specific primers shown in Supplementary Table1.The quantitative results of ChIP assay were normalized by the input control groups.Data are presented as mean ± SD from three independent experiments.***P ≤ 0.005; Two-tailed paired t test.f A reporter construct containing a p53-driven promoter sequence (pp53-TA-Luc) was transfected into MEFs.The thymidine kinase promoter-Renilla luciferase reporter plasmid (pRL-TK) was used as an internal control.Luciferase activities were determined at 16 h after transfection.Data are presented as mean ± SD from three independent experiments.***P ≤ 0.005; Two-tailed t test.g The protein expression of endogenous p21 Cip1/Waf1 (black arrow), p53 and WWOX and ectopic GFP-tagged human p53 (GFP-p53; black arrowhead) in late-passage Wwox +/+ , Wwox +/− , Wwox −/− MEFs (clone no.32-5, 54 and 56, respectively) was examined by western blotting.β-actin was used as an internal control.M.W., molecular weight.h Cell growth rates of late-passage Wwox +/+ , Wwox +/− , Wwox −/− (clone no.32-5, 54 and 56, respectively) and Wwox −/− MEFs expressing ectopic GFP-p53 or GFP protein.N.S. not significant; ***P ≤ 0.005; Two-tailed t test.i SA-βgal staining of late-passage Wwox +/+ , Wwox +/− , Wwox −/− (clone no.32-5, 54 and 56, respectively) and Wwox −/− MEFs expressing ectopic GFP-p53 protein.The lower panel pictures are the magnified images from the blue boxed areas in the upper panel.The percentages of SA-β-gal-positive senescent cells (red arrowheads) are shown at the right panel.Scale bars = 100 µm.*P ≤ 0.05; ***P ≤ 0.005; Two-tailed t test

338 Page 14 of 18 Fig. 6
Fig. 5 p53 inactivation causes the failure of senescence induction in Wwox −/− MEFs. a The upper panel is the schematic diagram of p53 protein.The amino acid sequence alignment of human and mouse p53 DBD is shown in the lower panel.TAD, transactivation domain; PRD, proline-rich domain; DBD, DNA-binding domain; TD, tetramerization domain; CRD, C-terminal regulatory domain.b, c The sequencing results of p53 gene coding region from Wwox +/− (clone no.54) and Wwox −/− (clone no.56) MEFs at passages 1 (early), 8 (intermediate) and 20 (late) are shown.In b, the nucleotide 638 from the translational start site of murine p53 changed from thymine to guanine, leading to a substitution of valine to glycine at position 213 in the intermediate and late-passage Wwox −/− MEF clone no.56.In c, the change of nucleotide 403 from guanine to cytosine resulted in a substitution of alanine to proline in p53 in the late-passage Wwox −/− MEF clone no.56.d Subcellular protein fractionation and western blotting were performed to examine the nuclear and cytosolic expression of p53 and p21 Cip1/Waf1 in latepassage Wwox +/− and Wwox −/− MEFs (clone no.54 and 56, respectively).Histone H3 and GAPDH were used as nuclear and cytosolic protein controls, respectively.e ChIP assay was conducted using the chromatin isolated from late-passage Wwox +/− and Wwox −/− MEFs (clone no.54 and 56, respectively).The immunoprecipitated chromatin using a control or anti-p53 antibody was analyzed for the presence of p21 Cip1/Waf1 promoter sequence (from − 200 to 98) by real-time PCR using the specific primers shown in Supplementary Table1.The quantitative results of ChIP assay were normalized by the input control groups.Data are presented as mean ± SD from three independent experiments.***P ≤ 0.005; Two-tailed paired t test.f A reporter construct containing a p53-driven promoter sequence (pp53-TA-Luc) was transfected into MEFs.The thymidine kinase promoter-Renilla luciferase reporter plasmid (pRL-TK) was used as an internal control.Luciferase activities were determined at 16 h after transfection.Data are presented as mean ± SD from three independent experiments.***P ≤ 0.005; Two-tailed t test.g The protein expression of endogenous p21 Cip1/Waf1 (black arrow), p53 and WWOX and ectopic GFP-tagged human p53 (GFP-p53; black arrowhead) in late-passage Wwox +/+ , Wwox +/− , Wwox −/− MEFs (clone no.32-5, 54 and 56, respectively) was examined by western blotting.β-actin was used as an internal control.M.W., molecular weight.h Cell growth rates of late-passage Wwox +/+ , Wwox +/− , Wwox −/− (clone no.32-5, 54 and 56, respectively) and Wwox −/− MEFs expressing ectopic GFP-p53 or GFP protein.N.S. not significant; ***P ≤ 0.005; Two-tailed t test.i SA-βgal staining of late-passage Wwox +/+ , Wwox +/− , Wwox −/− (clone no.32-5, 54 and 56, respectively) and Wwox −/− MEFs expressing ectopic GFP-p53 protein.The lower panel pictures are the magnified images from the blue boxed areas in the upper panel.The percentages of SA-β-gal-positive senescent cells (red arrowheads) are shown at the right panel.Scale bars = 100 µm.*P ≤ 0.05; ***P ≤ 0.005; Two-tailed t test ◂